This is 100% story mode, so that means all side missions and complete the main story. The longest side mission is Riddler, with 400 trophies in the game. I did the entire run off memory, my 2nd completed run without using my notes. I created a solid route a few months ago, and using it along with improv for political prisoners and zsasz, sub 4 is now possible. Catch out Cojosao if you want to see sub 4.